![]() ![]() As six tubes are used, the final reduction of germs/cells will be 10 -6 (1 in 1,000,000).Repeat the same process with the remaining test tube, taking 1 ml from the previous tube and adding to the next 9 ml diluents.The second tube now has a total dilution factor of 10 -2.Then dilute it and pour into a second test tube.Now, take 1 ml of mixture from the 10 -1 concentration test tube.Discharge a tip of pipette, and insert a new pipe tip into the pipette.Dilution is thoroughly mixed by removing and filling the pipette several times.This provides an initial dilution of 10 -1. Then add sample solution to the first tube to form a total volume of 10 ml.Add 1 ml of a well-mixed sample/culture is drawn into the pipette.Take a given sample/culture in a test tube and six test tubes, each containing 9 ml of sterile diluent, which can be pure water or 0.9% saline.The serial dilution formula for the dilution factor of each tube is –īeneath is an elucidated ten-step process designed to achieve a purification factor of 10 -6, providing an extensive understanding of every stage involved.The dilution factor in serial dilution can either be ascertained per test tube or taken into account as a collective diluting entity throughout the entire dilution series. ![]() ![]() Serial two-fold and ten-fold dilutions are commonly used to titer antibodies or prepare diluted analytes in the laboratory.On the other hand, for a less contaminated sample, a low dilution factor might be sufficient. For example, if a water sample is taken from a very polluted environment, the dilution factor is increased. Depending on the relative concentration of cells/organisms in the sample, the size of the stretch is determined.After that, a small average volume of each purifier is used to make a series of pouring or dispersing plates. A serial dilution is a series of dilutions made sequentially, using the same dilution factor for each step.Serial dilution involves the process of taking a sample and diluting it through a series of standard volumes of sterile diluent, which can be either distilled water or 0.9 % saline solution. ![]()
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